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| 基于MAPK途径探讨四物汤促进成骨细胞增殖的分子机制
摘要：目的：探究四物汤对小鼠胚胎成骨细胞前细胞（MC3T3-E1）增殖的影响及其经MAPK信号通路介导的分子作用机制。方法：将22日龄雌性SD大鼠20只随机分为四组：正常组，雌二醇组，四物汤高剂量组和四物汤低剂量组。四物汤经灌胃给药四天后，腹主动脉取血，制备含药血清。MTT法检测四物汤含药血清对MC3T3-E1细胞增殖的影响；Western blot法检测四物汤含药血清对MC3T3-E1细胞中MAPK信号通路相关蛋白ERK、p-ERK、p38、p-p38、JNK、p-JNK表达的调控作用。结果：MTT结果显示，与正常组相比，雌二醇及高、低剂量四物汤均能促进MC3T3-E1细胞增殖。Western blot结果表明，四物汤可上调MC3T3-E1细胞中ERK、p-ERK、p38、p-p38、JNK、p-JNK蛋白表达的水平。结论：四物汤可以促进成骨细胞MC3T3-E1增殖，并可能是通过上调MAPK信号通路实现的。
关键词：四物汤；成骨细胞；细胞增殖；MAPK信号通路
Molecular mechanism of Siwu Decoction containing serum promoting proliferation of osteoblasts based on MAPK signaling pathway
Abstract: Objective: To study the effect of Siwu Decoction on proliferation of mouse embryonic osteoblast precursor cells (MC3T3-E1) and explore its molecular mechanism via MAPK pathway. Methods: Twenty 22 days old female SD rats were randomly divided into four groups: normal group, estradiol group, high dose and low dose Siwu Decoction groups. After four days of administration of Siwu Decoction,blood was collected from abdominal aorta to prepare estradiol and Siwu
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