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| Rab9a 108位酪氨酸模拟磷酸化突变体的构建和功能初探 摘要 Rab蛋白是Ras蛋白家族中最大的一个家族，存在于全部真核生物，在进化过程中有高度的保守性，但在不同物种中又表现出数量和功能上的多样性。目前，人类已经发现约70种Rab蛋白和Rab样蛋白，其中Rab9是一种类似于ras的一类GTP酶（GTPase）,主要定位于晚期内含体[1],与甘露糖6-磷酸受体(MPR)从晚期内含体到反式高尔基网络的转运相关联。蛋白质磷酸化和去磷酸化是原核和真核生物细胞表达调控的关键环节,对许多生物的细胞功能起开关调控作用,是一种普遍的重要调节机制[2]。本实验拟通过构建磷酸化模拟突变体为Rab9a的磷酸化奠定前期基础，并通过分析其亚细胞定位，对其磷酸化功能进行初步探索,来设计抗病毒、治疗疾病的药物、调控Rab9a参与的自噬、吞噬等过程从而调节机体的免疫机能等等,现对其综述如下。 关键词：Ras超家族，Rab蛋白，Rab9蛋白，磷酸化 Construction and function of Rab9a 108 tyrosine mimic phosphorylation Mutant Abstract Rab protein is the largest family in the Ras protein family. It exists in all eukaryotes and has a high degree of conservation in the evolution process, but also shows a variety in number and function in different species. At present, about 70 Rab proteins and Rab like proteins have been found. Rab9 is a kind of GTP enzyme similar to ras, which is mainly located in late inclusions [1], and is associated with the transport of mannose 6-phosphate receptor (MPRs) from late inclusions to trans-Golgi networks. Protein phosphorylation and dephosphorylation are regulated by prokaryotic and eukaryotic cells It is a universal important regulatory mechanism that plays a switch role in regulating the cellular function of many organisms[2]. In this study, the phosphorylation mimic mutants were constructed to lay the foundation for the phosphorylation of Rab9a, and the phosphorylation function of Rab9a was preliminarily explored by analyzing its subcellular location to design antiviral and disease-resistant drugs. The regulation of autophagy, phagocytosis and other processes involved in Rab9a in order to regulate the immune function of the body is summarized as follows. Key words :Ras superfamily ,Rab protein,Rab9 protein,phosphorylation  |
